Thiolated chitosans: Are Cys-Cys ligands key to the next generation?

A Cys-Gly-Cys triad in the dehydrogenase region of Nox2 plays a key role in the interaction with p67phox.

p67(phox) is the paramount cytosolic regulator of the superoxide-generating Nox of phagocytes, by controlling the conformation of the catalytic component, Nox2. The initiating event of this process is a protein-protein interaction between p67(phox) and the part of Nox2 protruding into the cytosol, known as the dehydrogenase region. The aim of this study was to identify and characterize region(s...

Cys(9), Cys(104) and Cys(207) of simian virus 40 Vp1 are essential for infectious virion formation in CV-1 cells.

Structural studies have implicated Cys(9), Cys(104) and Cys(207) of simian virus 40 (SV40) Vp1 in disulfide bond formation. Recently, we have shown the three cysteines to be essential for disulfide linkage of Vp1 complexes in vitro. Here, the role of the three cysteines was explored during the course of SV40 infection. Single-, double- and triple-mutant Vp1 at Cys(9), Cys(104) and Cys(207) cont...

Conversion of Bacillus subtilis OhrR from a 1-Cys to a 2-Cys peroxide sensor.

OhrR proteins can be divided into two groups based on their inactivation mechanism: 1-Cys (represented by Bacillus subtilis OhrR) and 2-Cys (represented by Xanthomonas campestris OhrR). A conserved cysteine residue near the amino terminus is present in both groups of proteins and is initially oxidized to the sulfenic acid. The B. subtilis 1-Cys OhrR protein is subsequently inactivated by format...

Clxxxi. Dissociation Constants of Cys-

MATHEWS and WALKER [1909] and, in greater detail, Dixon and Tunnicliffe [1923] have demonstrated the great significance of the hydrogen ion activity in relation to the rates of oxidation and of autoxidation of a number of thioacids. To assist the analysis of these results and of certain observations of our own it became desirable to know the dissociation constants of cysteine, thioglycollic aci...

Middleware: the key to next generation computing